Composite
PcpcG2-HR

Part:BBa_K1642010:Design

Designed by: Can Yin   Group: iGEM15_SJTU-BioX-Shanghai   (2015-09-05)

Expression of Halorhodopsin under the control of PcpcG2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3398
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 966
    Illegal NgoMIV site found at 1125
    Illegal NgoMIV site found at 1446
    Illegal AgeI site found at 1317
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PcpcG2(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Promoters)

HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module)

PcpcG2-HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Desalination_Process)

Source

Overlap PCR from basic parts Up(BBa_K1642005), PcpcG2(BBa_K1642002), RBS(BBa_K1642004), HR(BBa_K1642001), Terminator(BBa_B0012), PcpcB-Kana(BBa_K1642003), Down(BBa_K1642006).